3A showed 70 differentially expressed genes (DEGs) including 13 flowering genes and sense FLAIL in flail3 versus Col-0 that were fully or partially rescued in the flail3 pFLAIL:gFLAIL18 complementation line (complement) for heatmap and hierarchical clustering. DEGs analyzed by DESeq2 with |log2 fold change| > 1 and adjusted p value < 0.05 were considered significant differential expression. Three biological replicates for flail3, wild type, and two for flail3 pFLAIL:gFLAIL18.
RNA-seq data is deposited on GEO with accession number GSE186212 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE186212) and codes used for DEG analysis is on github (https://github.com/Yu-Jin-KU/FLAIL_2021/blob/main/flail3_RNA_seq.R).
